Technician - Protein Production

Technician - Protein Production

For our client - a pioneering biotechnology company based in Groningen, The Netherlands - I am looking for a Technician - Protein Production to join their dynamic R&D team. This is a fantastic opportunity to contribute to novel proteomics and metabolomics technologies through hands-on protein expression and purification.

Key responsibilities:

• Perform heterologous protein expression in Escherichia coli using various expression systems.
• Purify proteins using techniques such as affinity chromatography (His-tag, GST-tag, etc.), ion exchange, and size-exclusion chromatography (SEC).
• Carry out molecular cloning workflows, including PCR, restriction digestion, ligation, and bacterial transformation.
• Analyze protein samples via SDS-PAGE and spectrophotometric assays.
• Maintain accurate and detailed laboratory records in compliance with standard operating procedures (SOPs) and Good Laboratory Practices (GLP).
• Assist in troubleshooting and optimizing expression and purification protocols.
• Support lab organization tasks, including reagent preparation, equipment maintenance, and inventory management.
• Collaborate with researchers, share findings, and contribute to project discussions.

Key requirements:

• Bachelor's or Master's degree in Biochemistry, Molecular Biology, Biotechnology, or a related field.
• Hands-on experience in protein expression and purification.
• Experience with FPLC (ÄKTA system) or other automated purification platforms is preferred.
• Familiarity with molecular biology techniques-especially cloning, PCR, and plasmid preparation.
• Strong analytical and problem-solving skills.
• Ability to follow standard protocols and maintain accurate documentation.
• Excellent organizational skills and attention to detail.

Practicalities:

• Location: Groningen, The Netherlands
• Permanent contract
• Start date: ASAP

Sound interesting?

Send your CV to Khanyi Mabena at or reach out directly at .